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anti cd47 antibody  (Bio X Cell)


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    Structured Review

    Bio X Cell anti cd47 antibody
    Anti Cd47 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd47+antibody/InVivoMAb+anti-mouse+human+rat+CD47/pm42901146-411-48-52
    Average 95 stars, based on 48 article reviews
    anti cd47 antibody - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Control:

    Article Title: Imiquimod enhances anti-tumor effects of CD47 targeting in oral squamous cell carcinoma.
    Article Snippet: Oral squamous cell carcinoma (OSCC) represents the most prevalent malignancy within oral cavity, which accounts for over 90% of all oral cancers [1, 2].. OSCC has become rising global burden, with an alarming fatality rate and 380,000 new cases annually [3].. Despite advancements in therapeutic interventions, the 5-year survival rate stays stagnant at roughly 50% for OSCC, highlighting the urgent need for innovative therapeutic strategies [4].

    Article Title: CD47 fuels intestinal fibrosis by inducing fibroblast phagocytic evasion and represents a therapeutic target.
    Article Snippet: .. For in vitro phagocytosis assays, primary intestinal fibroblasts were stimulated with TGF-β where indicated, labeled with CFSE (5 μM; eBioscience, 65–0850-84) for 20 min at room temperature, washed, and co-cultured with BMDMs for 24 h. For antibody-blockade assays, TGF-β-stimulated CFSE-labeled fibroblast-BMDM cultures were treated for 30 min with anti-CD47 antibody (20 μg/mL; Bio X Cell, BE0283), anti-SIRPα antibody (20 μg/mL; Bio X Cell, BE0322), or IgG control at co-culture initiation as appropriate for the experimental design. ..

    Incubation:

    Article Title: Imiquimod enhances anti-tumor effects of CD47 targeting in oral squamous cell carcinoma.
    Article Snippet: Oral squamous cell carcinoma (OSCC) represents the most prevalent malignancy within oral cavity, which accounts for over 90% of all oral cancers [1, 2].. OSCC has become rising global burden, with an alarming fatality rate and 380,000 new cases annually [3].. Despite advancements in therapeutic interventions, the 5-year survival rate stays stagnant at roughly 50% for OSCC, highlighting the urgent need for innovative therapeutic strategies [4].

    Flow Cytometry:

    Article Title: Imiquimod enhances anti-tumor effects of CD47 targeting in oral squamous cell carcinoma.
    Article Snippet: Oral squamous cell carcinoma (OSCC) represents the most prevalent malignancy within oral cavity, which accounts for over 90% of all oral cancers [1, 2].. OSCC has become rising global burden, with an alarming fatality rate and 380,000 new cases annually [3].. Despite advancements in therapeutic interventions, the 5-year survival rate stays stagnant at roughly 50% for OSCC, highlighting the urgent need for innovative therapeutic strategies [4].

    Analysis:

    Article Title: Imiquimod enhances anti-tumor effects of CD47 targeting in oral squamous cell carcinoma.
    Article Snippet: Oral squamous cell carcinoma (OSCC) represents the most prevalent malignancy within oral cavity, which accounts for over 90% of all oral cancers [1, 2].. OSCC has become rising global burden, with an alarming fatality rate and 380,000 new cases annually [3].. Despite advancements in therapeutic interventions, the 5-year survival rate stays stagnant at roughly 50% for OSCC, highlighting the urgent need for innovative therapeutic strategies [4].

    In Vitro:

    Article Title: CD47 fuels intestinal fibrosis by inducing fibroblast phagocytic evasion and represents a therapeutic target.
    Article Snippet: .. For in vitro phagocytosis assays, primary intestinal fibroblasts were stimulated with TGF-β where indicated, labeled with CFSE (5 μM; eBioscience, 65–0850-84) for 20 min at room temperature, washed, and co-cultured with BMDMs for 24 h. For antibody-blockade assays, TGF-β-stimulated CFSE-labeled fibroblast-BMDM cultures were treated for 30 min with anti-CD47 antibody (20 μg/mL; Bio X Cell, BE0283), anti-SIRPα antibody (20 μg/mL; Bio X Cell, BE0322), or IgG control at co-culture initiation as appropriate for the experimental design. ..

    Labeling:

    Article Title: CD47 fuels intestinal fibrosis by inducing fibroblast phagocytic evasion and represents a therapeutic target.
    Article Snippet: .. For in vitro phagocytosis assays, primary intestinal fibroblasts were stimulated with TGF-β where indicated, labeled with CFSE (5 μM; eBioscience, 65–0850-84) for 20 min at room temperature, washed, and co-cultured with BMDMs for 24 h. For antibody-blockade assays, TGF-β-stimulated CFSE-labeled fibroblast-BMDM cultures were treated for 30 min with anti-CD47 antibody (20 μg/mL; Bio X Cell, BE0283), anti-SIRPα antibody (20 μg/mL; Bio X Cell, BE0322), or IgG control at co-culture initiation as appropriate for the experimental design. ..

    Injection:

    Article Title: Methods and compositions for the treatment of cancer
    Article Snippet: .. For anti-CD47 antibody (clone MIAP410, BioXcell) treatment, the antibody was injected i.p. (400 g/mouse) starting on day 3 of tumor challenge and every third day thereafter. .. For PARP1 inhibitor treatment, olaparib (LC Laboratories) was dissolved in DMSO to 50 mg/ml.

    Blocking Assay:

    Article Title: Targeted Delivery of miR‐34a via Anti‐CD47 Antibody Conjugates for Enhanced Cancer Immunotherapy in Triple Negative Breast Cancer
    Article Snippet: Subsequently, the cellular uptake was assessed using a CytoFLEX flow cytometer (Beckman Coulter, USA). .. For blocking CD47, 4T1 cells were pre‐treated with anti‐CD47 antibody (1 mg mL −1 , BioXCell, USA) for 2 h to block CD47 on the cell membrane. .. For blocking CD47, 4T1 cells were pre‐treated with anti‐CD47 antibody (1 mg mL −1 , BioXCell, USA) for 2 h to block CD47 on the cell membrane.

    Membrane:

    Article Title: Targeted Delivery of miR‐34a via Anti‐CD47 Antibody Conjugates for Enhanced Cancer Immunotherapy in Triple Negative Breast Cancer
    Article Snippet: Subsequently, the cellular uptake was assessed using a CytoFLEX flow cytometer (Beckman Coulter, USA). .. For blocking CD47, 4T1 cells were pre‐treated with anti‐CD47 antibody (1 mg mL −1 , BioXCell, USA) for 2 h to block CD47 on the cell membrane. .. For blocking CD47, 4T1 cells were pre‐treated with anti‐CD47 antibody (1 mg mL −1 , BioXCell, USA) for 2 h to block CD47 on the cell membrane.

    Mouse Assay:

    Article Title: FACS-based genome-wide CRISPR screening platform identifies modulators of CD47
    Article Snippet: .. Mice with palpable tumors of similar size on day 5 were treated with anti-CD47 antibody (Bio X Cell, Cat. BE0270) in vivo . ..

    In Vivo:

    Article Title: FACS-based genome-wide CRISPR screening platform identifies modulators of CD47
    Article Snippet: .. Mice with palpable tumors of similar size on day 5 were treated with anti-CD47 antibody (Bio X Cell, Cat. BE0270) in vivo . ..



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    Real‐time monitoring of tumor progression and <t>anti‐CD47</t> therapeutic efficiency. (A) Female BALB/c mice aged 8–10 weeks were implanted with dorsal skinfold window chambers. 4T1_PB3R‐RFP TNBC cells were transplanted into the subcutaneous tissue, and tumors were monitored using intravital imaging. (B) Detailed visualization of angiogenesis and tumor nodule formation in TNBC skinfold models compared with the isotype control group. (C, D) Conventional whole‐body bioluminescence imaging of mice bearing 4T1_PB3R‐RFP‐luc tumors revealed considerably decreased signals following anti‐CD47 immunotherapy administered over 5 days, beginning on day 9 for 5 days ( n = 7, p < 0.0001, 10 mg/kg, intravenous injection). Data are presented as the mean ± standard deviation (SD).
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    a The synthetic process and the structure diagram of MAzyme (created in BioRender. Huang, H. (2026) https://BioRender.com/glo0g6v ). b TEM image of MAzyme and a magnification image (inset). c The high-resolution TEM image of MAzyme. d , e Elemental mapping images of MAzyme. f The structural changes of the initial and final states of the composite system in different models during the simulation process. g Temporal evolution of the self-aggregation supramolecular structure for MAzyme by MSD. h Fourier transform infrared (FTIR) spectrum of MAzyme. i High-resolution Cu 2 p X-ray photoelectron spectroscopy (XPS) results of MAzyme. j X-ray diffraction (XRD) pattern of MAzyme. k N 2 adsorption/desorption isotherms of MAzyme and the corresponding pore-size distribution curve calculated by non-local density functional theory (NLDFT, inset). l TEM image of biomimetic MMAzyme, indicating a cytomembrane layer coated on the surface of MAzyme. m Characteristic protein bands of CD36, <t>CD47,</t> and CCR2 on MAzyme, MMAzyme, and MM by Western blotting. n Zeta potentials and o particle diameter of MAzyme and MMAzyme analyzed by dynamic light scattering (DLS) ( n = 3 independent samples). Data in ( n , o ) were expressed as mean ± S.D. Source data are provided as a file.
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    Image Search Results


    Real‐time monitoring of tumor progression and anti‐CD47 therapeutic efficiency. (A) Female BALB/c mice aged 8–10 weeks were implanted with dorsal skinfold window chambers. 4T1_PB3R‐RFP TNBC cells were transplanted into the subcutaneous tissue, and tumors were monitored using intravital imaging. (B) Detailed visualization of angiogenesis and tumor nodule formation in TNBC skinfold models compared with the isotype control group. (C, D) Conventional whole‐body bioluminescence imaging of mice bearing 4T1_PB3R‐RFP‐luc tumors revealed considerably decreased signals following anti‐CD47 immunotherapy administered over 5 days, beginning on day 9 for 5 days ( n = 7, p < 0.0001, 10 mg/kg, intravenous injection). Data are presented as the mean ± standard deviation (SD).

    Journal: Cancer Medicine

    Article Title: In Vivo Immunoscoring Technique to Monitor Anti‐ CD47 Immunotherapy Efficacy in Triple‐Negative Breast Cancer

    doi: 10.1002/cam4.72232

    Figure Lengend Snippet: Real‐time monitoring of tumor progression and anti‐CD47 therapeutic efficiency. (A) Female BALB/c mice aged 8–10 weeks were implanted with dorsal skinfold window chambers. 4T1_PB3R‐RFP TNBC cells were transplanted into the subcutaneous tissue, and tumors were monitored using intravital imaging. (B) Detailed visualization of angiogenesis and tumor nodule formation in TNBC skinfold models compared with the isotype control group. (C, D) Conventional whole‐body bioluminescence imaging of mice bearing 4T1_PB3R‐RFP‐luc tumors revealed considerably decreased signals following anti‐CD47 immunotherapy administered over 5 days, beginning on day 9 for 5 days ( n = 7, p < 0.0001, 10 mg/kg, intravenous injection). Data are presented as the mean ± standard deviation (SD).

    Article Snippet: To evaluate the performance of the in vivo immunoscoring technique for the monitoring of anti‐CD47 immunotherapy efficacy, anti‐mouse CD47 monoclonal antibodies (specifically, anti‐CD47 mAbs, clone MIAP301, Rat IgG2a, κ, BioXcell, USA) and an IgG2a mAbs isotype control (clone 2A3, Rat IgG2a, κ, BioXcell, USA) were systemically and intravenously (IV) injected at a dosage of 10 mg/kg once a day for a duration of five consecutive days.

    Techniques: Real-time Monitoring, Tissue, Imaging, Control, Injection, Standard Deviation

    In vivo and in vitro validation of TNBC targeting by Dextran‐AF680. (A–C) The signals emitted by Dextran‐AF680 at various time intervals following intravenous injections exhibited significant differences between 10 and 180 min and between 180 and 360 min. (D, E) Dextran‐AF680 can be administrated for TNBC imaging with photoacoustic tomography, and the optimal imaging time is 3 h after dextran injection, indicating its potential as an imaging probe for enhancing TNBC visualization ( n = 7, p = 0.024, p = 0.004). (F, G) Longitudinal conventional fluorescence imaging of the anti‐CD47 immunotherapeutic response, in comparison with the isotype control treatment, demonstrated a significant change in the average radiant efficiency of Dextran AF680 following 5 days of treatment ( n = 12, p = 0.0022). (H, I) An evident shift in the population of Dextran‐AF680+ and RFP+ cells was observed after anti‐CD47 mAb immunotherapies ( n = 10, p = 0.0079). Data are presented as the mean ± standard deviation (SD).

    Journal: Cancer Medicine

    Article Title: In Vivo Immunoscoring Technique to Monitor Anti‐ CD47 Immunotherapy Efficacy in Triple‐Negative Breast Cancer

    doi: 10.1002/cam4.72232

    Figure Lengend Snippet: In vivo and in vitro validation of TNBC targeting by Dextran‐AF680. (A–C) The signals emitted by Dextran‐AF680 at various time intervals following intravenous injections exhibited significant differences between 10 and 180 min and between 180 and 360 min. (D, E) Dextran‐AF680 can be administrated for TNBC imaging with photoacoustic tomography, and the optimal imaging time is 3 h after dextran injection, indicating its potential as an imaging probe for enhancing TNBC visualization ( n = 7, p = 0.024, p = 0.004). (F, G) Longitudinal conventional fluorescence imaging of the anti‐CD47 immunotherapeutic response, in comparison with the isotype control treatment, demonstrated a significant change in the average radiant efficiency of Dextran AF680 following 5 days of treatment ( n = 12, p = 0.0022). (H, I) An evident shift in the population of Dextran‐AF680+ and RFP+ cells was observed after anti‐CD47 mAb immunotherapies ( n = 10, p = 0.0079). Data are presented as the mean ± standard deviation (SD).

    Article Snippet: To evaluate the performance of the in vivo immunoscoring technique for the monitoring of anti‐CD47 immunotherapy efficacy, anti‐mouse CD47 monoclonal antibodies (specifically, anti‐CD47 mAbs, clone MIAP301, Rat IgG2a, κ, BioXcell, USA) and an IgG2a mAbs isotype control (clone 2A3, Rat IgG2a, κ, BioXcell, USA) were systemically and intravenously (IV) injected at a dosage of 10 mg/kg once a day for a duration of five consecutive days.

    Techniques: In Vivo, In Vitro, Biomarker Discovery, Imaging, Micro-PAT, Injection, Fluorescence, Comparison, Control, Standard Deviation

    Intravital imaging of M2 macrophage targeting by Dextran‐AF680. (A) Dynamic in vivo distribution of intravenously injected Dextran‐AF680 in representative blood vessels of a skinfold TNBC model. The Dextran‐AF680 signal peaked at 3 h and dissipated from the vessels within 24 h. (B) Substantial colocalization was observed between Dextran‐AF680 targeted cells (red) and CD206‐FITC+ cells (green). (C) Antibody‐dependent cellular phagocytosis (ADCP) occured in rapid, brief waves between Dextran‐AF680‐labeled macrophages and CD47 inhibitor‐treated tumor cells in a skinfold TNBC model. (D) CD47 inhibitor‐treated cancer cells (green) were engulfed by macrophages labeled with the pan‐macrophage marker F4/80‐PE (red), underscoring the highly phagocytic nature of macrophages (scale bars, 25 μm). (E) Intravital imaging of the robust phagocytic capacity of F4/80+ macrophages (red) toward CD47 inhibitor‐treated tumor cells (green).

    Journal: Cancer Medicine

    Article Title: In Vivo Immunoscoring Technique to Monitor Anti‐ CD47 Immunotherapy Efficacy in Triple‐Negative Breast Cancer

    doi: 10.1002/cam4.72232

    Figure Lengend Snippet: Intravital imaging of M2 macrophage targeting by Dextran‐AF680. (A) Dynamic in vivo distribution of intravenously injected Dextran‐AF680 in representative blood vessels of a skinfold TNBC model. The Dextran‐AF680 signal peaked at 3 h and dissipated from the vessels within 24 h. (B) Substantial colocalization was observed between Dextran‐AF680 targeted cells (red) and CD206‐FITC+ cells (green). (C) Antibody‐dependent cellular phagocytosis (ADCP) occured in rapid, brief waves between Dextran‐AF680‐labeled macrophages and CD47 inhibitor‐treated tumor cells in a skinfold TNBC model. (D) CD47 inhibitor‐treated cancer cells (green) were engulfed by macrophages labeled with the pan‐macrophage marker F4/80‐PE (red), underscoring the highly phagocytic nature of macrophages (scale bars, 25 μm). (E) Intravital imaging of the robust phagocytic capacity of F4/80+ macrophages (red) toward CD47 inhibitor‐treated tumor cells (green).

    Article Snippet: To evaluate the performance of the in vivo immunoscoring technique for the monitoring of anti‐CD47 immunotherapy efficacy, anti‐mouse CD47 monoclonal antibodies (specifically, anti‐CD47 mAbs, clone MIAP301, Rat IgG2a, κ, BioXcell, USA) and an IgG2a mAbs isotype control (clone 2A3, Rat IgG2a, κ, BioXcell, USA) were systemically and intravenously (IV) injected at a dosage of 10 mg/kg once a day for a duration of five consecutive days.

    Techniques: Imaging, In Vivo, Injection, Labeling, Marker

    In vivo and in vitro validation of TNBC targeting by MCP1‐FITC. (A, B) Following intravenous injection of MCP1‐FITC, fluorescence signals in TNBC tissue peaked at 4 min and rapidly declined within 2 h ( n = 6, p = 0.0313). (C, D) Ex vivo imaging of dissected organs from subcutaneous xenograft models revealed a noticeable difference in fluorescence signal intensity between TNBC tumors and normal organs at 4 min after intravenous injection of MCP1‐FITC ( n = 6, p = 0.0095). (E, F) The average radiant efficiency of MCP1‐FITC in resected TNBC tissues was quantitively compared between the anti‐CD47 monoclonal antibody (mAb) and isotype control (ISO) treatment groups ( n = 16, p = 0.0499). (G) A significant difference in the M1/M2 ratio, based on the average radiant efficiency, was observed between anti‐CD47 mAb‐treated and ISO‐treated mice ( n = 12, p = 0.0022). (H) A pronounced shift in the population of MCP1‐FITC targeted cells was observed following repeated immunotherapy. (I, J) Significant differences in the M1/M2 ratio, based on the MCP‐1 FITC+ cells, is observed between anti‐CD47 mAb‐treated and ISO‐treated mice ( n = 16, p = 0.0104), ( n = 10, p = 0.0079). (K) A correlation analysis indicated that there is a strong correlation between the flow cytometry‐derived M1/M2 ratio and the imaging‐derived M1/M2 ratio. Data are presented as the mean ± standard deviation (SD).

    Journal: Cancer Medicine

    Article Title: In Vivo Immunoscoring Technique to Monitor Anti‐ CD47 Immunotherapy Efficacy in Triple‐Negative Breast Cancer

    doi: 10.1002/cam4.72232

    Figure Lengend Snippet: In vivo and in vitro validation of TNBC targeting by MCP1‐FITC. (A, B) Following intravenous injection of MCP1‐FITC, fluorescence signals in TNBC tissue peaked at 4 min and rapidly declined within 2 h ( n = 6, p = 0.0313). (C, D) Ex vivo imaging of dissected organs from subcutaneous xenograft models revealed a noticeable difference in fluorescence signal intensity between TNBC tumors and normal organs at 4 min after intravenous injection of MCP1‐FITC ( n = 6, p = 0.0095). (E, F) The average radiant efficiency of MCP1‐FITC in resected TNBC tissues was quantitively compared between the anti‐CD47 monoclonal antibody (mAb) and isotype control (ISO) treatment groups ( n = 16, p = 0.0499). (G) A significant difference in the M1/M2 ratio, based on the average radiant efficiency, was observed between anti‐CD47 mAb‐treated and ISO‐treated mice ( n = 12, p = 0.0022). (H) A pronounced shift in the population of MCP1‐FITC targeted cells was observed following repeated immunotherapy. (I, J) Significant differences in the M1/M2 ratio, based on the MCP‐1 FITC+ cells, is observed between anti‐CD47 mAb‐treated and ISO‐treated mice ( n = 16, p = 0.0104), ( n = 10, p = 0.0079). (K) A correlation analysis indicated that there is a strong correlation between the flow cytometry‐derived M1/M2 ratio and the imaging‐derived M1/M2 ratio. Data are presented as the mean ± standard deviation (SD).

    Article Snippet: To evaluate the performance of the in vivo immunoscoring technique for the monitoring of anti‐CD47 immunotherapy efficacy, anti‐mouse CD47 monoclonal antibodies (specifically, anti‐CD47 mAbs, clone MIAP301, Rat IgG2a, κ, BioXcell, USA) and an IgG2a mAbs isotype control (clone 2A3, Rat IgG2a, κ, BioXcell, USA) were systemically and intravenously (IV) injected at a dosage of 10 mg/kg once a day for a duration of five consecutive days.

    Techniques: In Vivo, In Vitro, Biomarker Discovery, Injection, Fluorescence, Tissue, Ex Vivo, Imaging, Xenograft Assay, Control, Analysis, Flow Cytometry, Derivative Assay, Standard Deviation

    a The synthetic process and the structure diagram of MAzyme (created in BioRender. Huang, H. (2026) https://BioRender.com/glo0g6v ). b TEM image of MAzyme and a magnification image (inset). c The high-resolution TEM image of MAzyme. d , e Elemental mapping images of MAzyme. f The structural changes of the initial and final states of the composite system in different models during the simulation process. g Temporal evolution of the self-aggregation supramolecular structure for MAzyme by MSD. h Fourier transform infrared (FTIR) spectrum of MAzyme. i High-resolution Cu 2 p X-ray photoelectron spectroscopy (XPS) results of MAzyme. j X-ray diffraction (XRD) pattern of MAzyme. k N 2 adsorption/desorption isotherms of MAzyme and the corresponding pore-size distribution curve calculated by non-local density functional theory (NLDFT, inset). l TEM image of biomimetic MMAzyme, indicating a cytomembrane layer coated on the surface of MAzyme. m Characteristic protein bands of CD36, CD47, and CCR2 on MAzyme, MMAzyme, and MM by Western blotting. n Zeta potentials and o particle diameter of MAzyme and MMAzyme analyzed by dynamic light scattering (DLS) ( n = 3 independent samples). Data in ( n , o ) were expressed as mean ± S.D. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Bioinspired porous metal-amino acid nanozymes enable multi-pathway treatment of atherosclerosis through anti-oxidation and pro-efferocytosis

    doi: 10.1038/s41467-026-75509-4

    Figure Lengend Snippet: a The synthetic process and the structure diagram of MAzyme (created in BioRender. Huang, H. (2026) https://BioRender.com/glo0g6v ). b TEM image of MAzyme and a magnification image (inset). c The high-resolution TEM image of MAzyme. d , e Elemental mapping images of MAzyme. f The structural changes of the initial and final states of the composite system in different models during the simulation process. g Temporal evolution of the self-aggregation supramolecular structure for MAzyme by MSD. h Fourier transform infrared (FTIR) spectrum of MAzyme. i High-resolution Cu 2 p X-ray photoelectron spectroscopy (XPS) results of MAzyme. j X-ray diffraction (XRD) pattern of MAzyme. k N 2 adsorption/desorption isotherms of MAzyme and the corresponding pore-size distribution curve calculated by non-local density functional theory (NLDFT, inset). l TEM image of biomimetic MMAzyme, indicating a cytomembrane layer coated on the surface of MAzyme. m Characteristic protein bands of CD36, CD47, and CCR2 on MAzyme, MMAzyme, and MM by Western blotting. n Zeta potentials and o particle diameter of MAzyme and MMAzyme analyzed by dynamic light scattering (DLS) ( n = 3 independent samples). Data in ( n , o ) were expressed as mean ± S.D. Source data are provided as a file.

    Article Snippet: The following primary antibodies were used in this study: CD47 Rabbit pAb (A1838, ABclonal,1:750), CD36/SR-B3 Rabbit pAb (A5792, ABclonal,1:2500), CCR2 Rabbit pAb (A2855, ABclonal,1:750), Na-K-ATP Rabbit pAb (GB11400, Servicebio,1:2000), MERTK Rabbit pAb (A5443, ABclonal,1:750), LXRα Rabbit mAb (A3974, ABclonal,1:3000), ABCG1 Rabbit mAb (A17907, ABclonal,1:1500), PPARγ Rabbit pAb (WL01800, Wanleibio,1:1000), Anti-ABCA1 antibody ( AB307536 , Abcam,1:1000), GAPDH Rabbit pAb (AC001, ABclonal,1:20000), p-SHP1 (8849, Cell Signaling Technology, 1:1000), HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014, ABclonal,1:5000).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Adsorption, Pore Size, Functional Assay, Western Blot

    a Schematic illustration of the pro-efferocytosis property of MMAzyme-S. (Created in BioRender. Huang, H. (2026) https://BioRender.com/7l5dwq4 ). b CLSM images depicting phagocytosis of CD47-overexpressed ACs in proinflammatory-phenotype macrophages pretreated with different samples. FCM results ( c ) and the corresponding quantitative analysis ( d ) of efferocytosis towards CD47-overexpressed ACs by proinflammatory phenotype macrophages pretreated with different samples ( n = 3 independent biological samples). e The quantitative results of the Western blotting of Mertk in macrophages under different treatments ( n = 3 independent biological samples. Samples in different blots were derived from the same experiment. All gels/blots were processed in parallel. f Schematics illustration for the mechanism of promoting cholesterol efflux mediated by MMAzyme-S (created in BioRender. Huang, H. (2026) https://BioRender.com/o3x56t5 ). g Optical microscopy images of induced foamy cell formation in macrophages under different treatments. h Cholesterol content in the supernatant of foamy cells under different treatments ( n = 4 independent biological samples). i Western blotting analysis of Mertk, PPARγ, LXRα, ABCA1, and ABCG1 in macrophages under different treatments. Corresponding quantitative results of the Western blotting of PPARγ ( j ), LXRα ( k ), ABCA1 ( l ), and ABCG1 ( m ) in macrophages under different treatments ( n = 3 independent biological samples. Samples in different blots were derived from the same experiment. All gels/blots were processed in parallel). Data in ( d , e , h , j – m ) were expressed as mean ± S.D. Significance in ( d , e , h , j – m ) was calculated using one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. Although the individual values of Group ii and Group iii in ( l ) do not overlap, the statistical analysis does not indicate a significant difference, possibly due to the limited sample size. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Bioinspired porous metal-amino acid nanozymes enable multi-pathway treatment of atherosclerosis through anti-oxidation and pro-efferocytosis

    doi: 10.1038/s41467-026-75509-4

    Figure Lengend Snippet: a Schematic illustration of the pro-efferocytosis property of MMAzyme-S. (Created in BioRender. Huang, H. (2026) https://BioRender.com/7l5dwq4 ). b CLSM images depicting phagocytosis of CD47-overexpressed ACs in proinflammatory-phenotype macrophages pretreated with different samples. FCM results ( c ) and the corresponding quantitative analysis ( d ) of efferocytosis towards CD47-overexpressed ACs by proinflammatory phenotype macrophages pretreated with different samples ( n = 3 independent biological samples). e The quantitative results of the Western blotting of Mertk in macrophages under different treatments ( n = 3 independent biological samples. Samples in different blots were derived from the same experiment. All gels/blots were processed in parallel. f Schematics illustration for the mechanism of promoting cholesterol efflux mediated by MMAzyme-S (created in BioRender. Huang, H. (2026) https://BioRender.com/o3x56t5 ). g Optical microscopy images of induced foamy cell formation in macrophages under different treatments. h Cholesterol content in the supernatant of foamy cells under different treatments ( n = 4 independent biological samples). i Western blotting analysis of Mertk, PPARγ, LXRα, ABCA1, and ABCG1 in macrophages under different treatments. Corresponding quantitative results of the Western blotting of PPARγ ( j ), LXRα ( k ), ABCA1 ( l ), and ABCG1 ( m ) in macrophages under different treatments ( n = 3 independent biological samples. Samples in different blots were derived from the same experiment. All gels/blots were processed in parallel). Data in ( d , e , h , j – m ) were expressed as mean ± S.D. Significance in ( d , e , h , j – m ) was calculated using one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. Although the individual values of Group ii and Group iii in ( l ) do not overlap, the statistical analysis does not indicate a significant difference, possibly due to the limited sample size. Source data are provided as a file.

    Article Snippet: The following primary antibodies were used in this study: CD47 Rabbit pAb (A1838, ABclonal,1:750), CD36/SR-B3 Rabbit pAb (A5792, ABclonal,1:2500), CCR2 Rabbit pAb (A2855, ABclonal,1:750), Na-K-ATP Rabbit pAb (GB11400, Servicebio,1:2000), MERTK Rabbit pAb (A5443, ABclonal,1:750), LXRα Rabbit mAb (A3974, ABclonal,1:3000), ABCG1 Rabbit mAb (A17907, ABclonal,1:1500), PPARγ Rabbit pAb (WL01800, Wanleibio,1:1000), Anti-ABCA1 antibody ( AB307536 , Abcam,1:1000), GAPDH Rabbit pAb (AC001, ABclonal,1:20000), p-SHP1 (8849, Cell Signaling Technology, 1:1000), HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014, ABclonal,1:5000).

    Techniques: Western Blot, Derivative Assay, Microscopy